Nephroprotection ,Lophira lanceolata ,Gentamicin ,

In spite of the efforts to improve the care of renal failure, its prevalence is still increasing. Regarding the cost of dialysis, populations have resort to numerous traditional medicine recipes whose efficiency and safety are not always clear. This study aims to assess the nephroprotective effects of the aqueous extract of Lophira lanceolata’s leaves in albino wistar rats poisoned with gentamicin. To achieve this, we first determine the major molecule families present in the extract followed by the demonstration of its safety through the evaluation of the toxicity up to the dose limit of 2000 mg/kg of body weight. Last but not the least; we assessed the biological activity of the extract on an acute renal failure provoked in Wistar rats. Thereby, 30 male rats of 200 g average weight were distributed in three (03) lots of ten (10) including one control group. Gentamicin (80 mg/kg/day) was then injected by intra-peritoneal route for 7 days to the rats of the second lot. In addition to gentamicin, the rats of the third lot received the aqueous extract (1000 mg/Kg/day) for 7 days. Blood, urine and organs (liver, kidney) samples were collected from the rats after treatment respectively for the dosage of urea and creatinine; the clearance of creatinine and the histological analysis. Gentamicin led to an elevation of the uremia (184.70 ± 28.77 mg/dl) and of the creatininemia (1.42 ± 0.06 mg/dl); a considerable reduction of the clearance of creatinine (19.60 ± 5.21 ml/hrs) and a necrosis of the epithelial cells of the proximal tubules (P <0.05). It comes out of this study that the aqueous extract of Lophira lanceolata’s leaves reduced significantly the nephrotoxic effects of gentamicine.

5 , 2 , 2015