Department of Pharmacology, Geethanjali college of Pharmacy, Cherryal (V), R.R(Dist), Telangana, India-501301.

Clinical Pharmacology, Aizant pharmaceuticals, Telangana, India-501301.
MDR1 ,PCR ,Polymorphism ,Cervical cancer ,Toxicity ,

Polymorphisms in genes coding for metabolizing enzymes can affect drug efficacy and toxicity. The cervical cancer is the leading cause of death from gynecological malignancy, and the second most commonly diagnosed gynecologic malignancy. The present study will be undertaken with an objective to evaluate the clinical significance of polymorphisms in drug-metabolizing enzymes in cervical cancer patients. Genomic DNA isolation will do for control blood samples and homogenised tumor samples by salting out method followed by ethanol extraction. Agarose gel electrophoresis was presence of DNA well cheked by using 0.8% agarose gel electrophoresis. Polymerase chain reaction (PCR) amplification will be done by using specific set of primers and PCR condition followed by agarose gel electrophoresis to conform the amplified product, Single strand confirmatory polymorphisms (SSCP) of the PCR product will done for detecting the presence of polymorphisms in the amplified exons from cancer samples compared to healthy samples, Sequencing: Sequence analysis will done for the strands which showing shifts in SSCP. Detection of single nucleotide polymorphisms in Cervical cancer patients lead to safer chemotherapeutic procedures for their treatment, and play role in Disease prognosis, relative risk individually in developing cervical cancer. Hence this study forms the basis of an effort to reduce the trauma and suffering of the cervical cancer patient

5 , 2 , 2015