Department of Microbiology and Food technology, BFIT Group of Institutions, Dehradun 248 007, Uttarakhand, India. 5

Department of Botany, BFIT Group of Institutions, Dehradun 248 007, Uttarakhand, India. 5

Department of Zoology,BFIT Group of Institutions, Dehradun 248 007, Uttarakhand, India. 5

Department of Chemistry, BFIT Group of Institutions, Dehradun 248 007, Uttarakhand, India.

Department of Microbiology, Doon (P.G.) College of Agriculture Science and technology, Dehradun, Uttarakhand, India.

Department of Microbiology, Doon (P.G.) Paramedical Colleg
Murraya koenigii ,Klebsiella pneumoniae ,Norfloxacin ,Synergism ,Antimicrobial activity ,

Klebsiella pneumoniae strains is one of the UTI causing bacteria. So 32 UTI samples were collected and characterized on the basis of biochemical tests i.e. Bergey’s manual of determinative bacteriology [14]. Only, 7 UTI positive Klebsiella pneumoniae were selected for present study. Murraya koenigii is medicinal plant and evaluated the antibacterial activity of aqueous extract of M. koenigii. Preparation of extraction of leaves of the M. koenigii and preparation of various treatments of M. koenigii and Norfloxacin were prepared by standard methods. Stock concentration of extract of the Murraya koenigii and Norfloxacin was 50µg/ml. 5 treatments were prepared and observed that maximum inhibition zone was observed in treatment (50% medicinal plant + 50 % antibiotics) i.e. 43 mm. Least inhibition zone has been seen in treatment 1 (100% medicinal plant) i.e. 21 mm. Our results suggested that 7 isolated strains were Klebsiella pneumoniae and antimicrobial study revealed that 50% aqueous extract of Murraya koenigii and 50% Norfloxacin showed highest antibacterial activity against Klebsiella pneumoniae.

4 , 3 , 2014