Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda-508284, Andhra Pradesh, India.

Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda-508284, Andhra Pradesh, India.

Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda-508284, Andhra Pradesh, India.

Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda-508284, Andhra Pradesh, India.

Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda-508284, Andhra Pradesh, India.
Ocimum sanctum ,Anti-diabetic , Acute oral toxicity ,

The present study was undertaken to evaluate the anti-diabetic activity of Ocimum sanctum in alloxan induced diabetic rats. The currently available drug regimens for management of diabetes mellitus have certain drawbacks and therefore there is a need to find safer and more effective anti-diabetic drugs. The present study was carried out to evaluate the antidiabetogenic effect of ethanolic extract of roots of Ocimum sanctum in alloxan-induced diabetic rats in dose dependent manner. Alloxan injection resulted in diabetes mellitus which may be due to destruction of β cells of Islets of Langerhans. The crude ethanolic extract of roots of Ocimum sanctum were subjected to preliminary phytochemical analysis which shows the presence of carbohydrates, flavonoids, sterols, alkaloids, amino acids which are potent antioxidant and have pronounced pharmacological action. Oral administration of the extract (200&400mg/kg body weight) for 15 days significantly (p<0.05) lowered the hyperglycaemia of the experimental groups in respect to diabetic rats. Among the two doses of extract 400mg/kg dose showed significant anti-hyperglycaemic effect showing positive results of the extract. Acute oral toxicity study of roots of Ocimum sanctum did not exhibit any lethality or any profound toxic reactions at a dose of 2000mg/kg per oral. According to the (OECD) 423 guidelines for acute oral toxicity study LD50 dose of 2000mg/kg per oral of roots of Ocimum sanctum is found to be safe.

4 , 1 , 2014