Department of Pharmacology, St. Soldier Institute of Pharmacy, Lidhran Campus, Behind NIT (R.E.C.), Jalandhar – Amritsar by pass, NH-1, Jalandhar -144011, Punjab, India

Department of Pharmacology, St. Soldier Institute of Pharmacy, Lidhran Campus, Behind NIT (R.E.C.), Jalandhar – Amritsar by pass, NH-1, Jalandhar -144011, Punjab, India
Nyctanthesarbor-tristis, Anxiolytic, corticosterone levels, LDM – Light Dark Model, EPM – elevated plus maze ,

Aim: To evaluate Anxiolytic Activity of Aqueous and Ethanolic extracts from leaves of Nyctanthes arbor-tristisin albino mice by using Elevated plus Maze (EPM) and Light Dark Model (LDM) parameters. Materials and Methods: Aqueous and ethanolic extract of leaves of Nyctanthes arbor-tristis (250mg/kg, 500mg/kg), diazepam 1mg/kg are administered orally to randomly divided albino mice of either sex. 36 healthy albino mice of either sex were randomly divided into 6 groups of 6 each (n=6), weighing about 25-30 grams were selected for the study. Results: Anxiolytic activity is assessed by Elevated Plus Maze (EPM) and Light Dark Model (LDM) for locomotor activity. In EPM, treatment with ethanolic and aqueous extracts increased the time spent in open arm and total locomotion time. In light dark model treatment with these extracts showed increase in time spent in light compartment. Leaf extract (LE) at doses 250mg/kg and 500mg/kg have shown significant anxiolytic activity. Conclusion The plant Nyctanthes arbor tristis's anxiolytic activity against the EPM and LDM, the ethanolic extract, the aqueous extract had fewer effects. While ethanol extract demonstrated some degree of anxiolytic efficacy, this may have been caused by the active ingredients' insoluble nature in aqueous solutions

15 , 1 , 2025