Research Scholar, OPJS University, Rajgarh, Churu, Sadulpur,Rajasthan, 331303, India.

Research Guide, OPJS University, Rajgarh, Churu, Sadulpur,Rajasthan, 331303, India.
Chronic toxicity ,Body weight ,Feed intake ,Clinical biochemistry ,Heamatology ,

ABSTRACT This study investigated the acute and chronic toxicity of leaf aqueous extract of Desmodium triflorum (AEDT) in rats for evaluating its safety profile. AEDT for the acute (upto 2000 mg/kg) and chronic (200, 1000 and 2000 mg/kg) toxicity studies was administered orally to rats according the guidelines 425 and 407 of Organization for Economic Cooperation and Development, respectively. Food and water intake as well as body weight of animals were recorded. Physical and neurological signs of toxicity were assessed, and hematological, biochemical and histopathological analyses were performed. In the acute toxicity study, a single dose of the aqueous extract at 2000 mg/kg caused no mortality in the animals, suggesting that the median lethal dose is greater than 2000 mg/kg. In the chronic toxicity study, administration of the extract for 91 days, at all doses caused no significant changes in the body weights or organ weights of rats in the treated groups when compared with the control group. In addition, hematological and biochemical parameters also revealed no toxic effects of the extract on rats. Histological sections of the all organ from test animals showed no signs of degeneration except liver show mild changes at 2000 mg/kg dose. These results showed that AEDT at dosage levels up to 2000 mg/kg is nontoxic and could also offer protection on some body tissues. AEDT could, therefore, be considered safe

9 , 2 , 2019