Department of pharmaceutical chemistry, J.K.K.Munirajah Medical Research Foundation, College of pharmacy, Komarapalayam, Tamilnadu, India.

Department of pharmaceutical chemistry, J.K.K.Munirajah Medical Research Foundation, College of pharmacy, Komarapalayam, Tamilnadu, India.
Carissa spinarum L ,Cytotoxicity Activity ,MTT Assay ,SRB Assay ,Ehrlich Ascites Carcinoma ,Daltons Lymphoma Asties ,Hella cell line ,

Since many years plants were known to possess anticancer activities against different cancer cell lines. In this paper ,we report a study based on anticancer properties of Carissa spinarum L. The aerial parts of plant material were collected, shade dried and extracted with pet ether , chloroform, ethyl acetate, and ethanol using soxhlet extraction procedure. The study was aimed to evaluation of the anticancer activity of the ethanolic fractions of Carissa spinarum L have been traditionally used for many ailments including cancer. In the present study, anticancer activity of ethanolic fractions of Carissa spinarum L was evaluated using different invitro methods. In vitro antitumor activity was evaluated by the MTT assay and SRB method using Vero and HEP-2 cell lines. . In in vitro activity plant fractions exhibited significant cytotoxic activity against both cells lines even at different concentrations. Oral administration of CSF at the dose of 250,500 and 1000 mg/kg, The results showed the significant decrease of the viability of the cells in a concentration dependent manner.

6 , 2 , 2016